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gotag qpcr master mix #a6001  (Promega)

 
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    Structured Review

    Promega gotag qpcr master mix #a6001
    A The mRNA expression levels in cerebellar slice cultures of heme oxygenase 1 ( Hmox1 ), glutathione peroxidase 2 ( Gpx2 ), Glucose-6-phosphate dehydrogenase ( G6pd ), and glutamate cysteine ligase catalytic subunit ( Gclc ) were investigated after OGD with and without treatment with MMF for 24 h by <t>qPCR.</t> B The mRNA expression levels of the appropriate genes in hippocampal slice cultures were investigated after OGD with and without treatment with MMF for 24 h by qPCR. For relative quantification, the 2 −ΔΔCt method was performed using actin ACTB for normalization. Gene expression was investigated in triplicates. FC = Fold Change; n = 4–6. N = 3. All data are provided as mean ± SEM. Data were tested for significance using Student‘s t-test. Significant differences are indicated by *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. For non-significant changes in Gpx2 expression in hippocampal slice cultures, p-values have been included to illustrate the observed trend.
    Gotag Qpcr Master Mix #A6001, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gotag+qpcr+master+mix/pmc11305562-100-0-5?v=Promega
    Average 90 stars, based on 1 article reviews
    gotag qpcr master mix #a6001 - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Region-specific protective effects of monomethyl fumarate in cerebellar and hippocampal organotypic slice cultures following oxygen-glucose deprivation"

    Article Title: Region-specific protective effects of monomethyl fumarate in cerebellar and hippocampal organotypic slice cultures following oxygen-glucose deprivation

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0308635

    A The mRNA expression levels in cerebellar slice cultures of heme oxygenase 1 ( Hmox1 ), glutathione peroxidase 2 ( Gpx2 ), Glucose-6-phosphate dehydrogenase ( G6pd ), and glutamate cysteine ligase catalytic subunit ( Gclc ) were investigated after OGD with and without treatment with MMF for 24 h by qPCR. B The mRNA expression levels of the appropriate genes in hippocampal slice cultures were investigated after OGD with and without treatment with MMF for 24 h by qPCR. For relative quantification, the 2 −ΔΔCt method was performed using actin ACTB for normalization. Gene expression was investigated in triplicates. FC = Fold Change; n = 4–6. N = 3. All data are provided as mean ± SEM. Data were tested for significance using Student‘s t-test. Significant differences are indicated by *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. For non-significant changes in Gpx2 expression in hippocampal slice cultures, p-values have been included to illustrate the observed trend.
    Figure Legend Snippet: A The mRNA expression levels in cerebellar slice cultures of heme oxygenase 1 ( Hmox1 ), glutathione peroxidase 2 ( Gpx2 ), Glucose-6-phosphate dehydrogenase ( G6pd ), and glutamate cysteine ligase catalytic subunit ( Gclc ) were investigated after OGD with and without treatment with MMF for 24 h by qPCR. B The mRNA expression levels of the appropriate genes in hippocampal slice cultures were investigated after OGD with and without treatment with MMF for 24 h by qPCR. For relative quantification, the 2 −ΔΔCt method was performed using actin ACTB for normalization. Gene expression was investigated in triplicates. FC = Fold Change; n = 4–6. N = 3. All data are provided as mean ± SEM. Data were tested for significance using Student‘s t-test. Significant differences are indicated by *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. For non-significant changes in Gpx2 expression in hippocampal slice cultures, p-values have been included to illustrate the observed trend.

    Techniques Used: Expressing, Quantitative Proteomics, Gene Expression



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    A The mRNA expression levels in cerebellar slice cultures of heme oxygenase 1 ( Hmox1 ), glutathione peroxidase 2 ( Gpx2 ), Glucose-6-phosphate dehydrogenase ( G6pd ), and glutamate cysteine ligase catalytic subunit ( Gclc ) were investigated after OGD with and without treatment with MMF for 24 h by <t>qPCR.</t> B The mRNA expression levels of the appropriate genes in hippocampal slice cultures were investigated after OGD with and without treatment with MMF for 24 h by qPCR. For relative quantification, the 2 −ΔΔCt method was performed using actin ACTB for normalization. Gene expression was investigated in triplicates. FC = Fold Change; n = 4–6. N = 3. All data are provided as mean ± SEM. Data were tested for significance using Student‘s t-test. Significant differences are indicated by *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. For non-significant changes in Gpx2 expression in hippocampal slice cultures, p-values have been included to illustrate the observed trend.
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    A The mRNA expression levels in cerebellar slice cultures of heme oxygenase 1 ( Hmox1 ), glutathione peroxidase 2 ( Gpx2 ), Glucose-6-phosphate dehydrogenase ( G6pd ), and glutamate cysteine ligase catalytic subunit ( Gclc ) were investigated after OGD with and without treatment with MMF for 24 h by <t>qPCR.</t> B The mRNA expression levels of the appropriate genes in hippocampal slice cultures were investigated after OGD with and without treatment with MMF for 24 h by qPCR. For relative quantification, the 2 −ΔΔCt method was performed using actin ACTB for normalization. Gene expression was investigated in triplicates. FC = Fold Change; n = 4–6. N = 3. All data are provided as mean ± SEM. Data were tested for significance using Student‘s t-test. Significant differences are indicated by *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. For non-significant changes in Gpx2 expression in hippocampal slice cultures, p-values have been included to illustrate the observed trend.
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    Image Search Results


    A The mRNA expression levels in cerebellar slice cultures of heme oxygenase 1 ( Hmox1 ), glutathione peroxidase 2 ( Gpx2 ), Glucose-6-phosphate dehydrogenase ( G6pd ), and glutamate cysteine ligase catalytic subunit ( Gclc ) were investigated after OGD with and without treatment with MMF for 24 h by qPCR. B The mRNA expression levels of the appropriate genes in hippocampal slice cultures were investigated after OGD with and without treatment with MMF for 24 h by qPCR. For relative quantification, the 2 −ΔΔCt method was performed using actin ACTB for normalization. Gene expression was investigated in triplicates. FC = Fold Change; n = 4–6. N = 3. All data are provided as mean ± SEM. Data were tested for significance using Student‘s t-test. Significant differences are indicated by *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. For non-significant changes in Gpx2 expression in hippocampal slice cultures, p-values have been included to illustrate the observed trend.

    Journal: PLOS ONE

    Article Title: Region-specific protective effects of monomethyl fumarate in cerebellar and hippocampal organotypic slice cultures following oxygen-glucose deprivation

    doi: 10.1371/journal.pone.0308635

    Figure Lengend Snippet: A The mRNA expression levels in cerebellar slice cultures of heme oxygenase 1 ( Hmox1 ), glutathione peroxidase 2 ( Gpx2 ), Glucose-6-phosphate dehydrogenase ( G6pd ), and glutamate cysteine ligase catalytic subunit ( Gclc ) were investigated after OGD with and without treatment with MMF for 24 h by qPCR. B The mRNA expression levels of the appropriate genes in hippocampal slice cultures were investigated after OGD with and without treatment with MMF for 24 h by qPCR. For relative quantification, the 2 −ΔΔCt method was performed using actin ACTB for normalization. Gene expression was investigated in triplicates. FC = Fold Change; n = 4–6. N = 3. All data are provided as mean ± SEM. Data were tested for significance using Student‘s t-test. Significant differences are indicated by *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. For non-significant changes in Gpx2 expression in hippocampal slice cultures, p-values have been included to illustrate the observed trend.

    Article Snippet: GoTag qPCR Master Mix (#A6001, Promega, USA) was used with 50 ng cDNA and the corresponding primer sets (0.7 μM each).

    Techniques: Expressing, Quantitative Proteomics, Gene Expression